Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 May 21;7(8):e202402740. doi: 10.26508/lsa.202402740

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2024 Waltho et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Figure S12. — (A) Overlaid affinity plots of triplicate measurements of K48 Ub2, K48 Ub3, and mono-Ub interacting with immobilized biotinylated HIP1(22-309) protein. Averaged response values (RU) at equilibrium are plotted against the injected concentration (μM) of respective analytes determined by surface plasmon resonance multicycle format and fitted according to a steady-state affinity model. (B, C, D, E, F, G) Sensorgrams of (B) mono-Ub, (C) K48 Ub2, (D) K48 Ub3, (E) K63 Ub2, (F) K63 Ub3, and (G) Br Ub3 binding to immobilized HIP1(22-309), by surface plasmon resonance. Response values (RU) are plotted against time in seconds.