Figure 6.

Global genetic deficiency of Gdf15 alters the lung transcriptional profile during lung injury elicited by intratracheal instillation of P. aeruginosa exoproducts. WT mice (n = 5) and mice globally deficient for GDF15 (Gdf15^−/−; n = 5) were intratracheally inoculated with PA SN, and RNA was collected 20 hours after infection. (A) Volcano plot of normalized RNA counts with annotations showing 3,005 differentially expressed genes with adjusted P value <0.05. (B) Heat map of top differentially expressed genes after regularized log transformation of RNA counts and removal of pseudogenes clustered by row with genotype by column. (C and D) Dot plots of overrepresentation analysis for Gene Ontology molecular functions (P < 0.01, q < 0.05) in the 20 most upregulated (C) and downregulated (D) gene sets using all genes with statistically significant differential RNA counts (adjusted P < 0.05). (E) Gene set enrichment analysis of Hallmark pathways (adjusted P < 0.05) of all transcripts from control and Gdf15^−/− mice. (F and G) CXCL10 was quantified by ELISA in lung homogenate (F) and BAL fluid (G). Each dot represents a single mouse. Groups were compared using a Mann-Whitney test.