Figure 2. Graphical overview for the single cell RNA sequencing analysis workflow described in this manuscript.

Starting from a matrix of expression counts, we use the scater and scran Bioconductor packages to perform QC and initial exploratory analyses. To robustly quantify transcriptional heterogeneity within seemingly homogeneous cell populations, we apply the BASiCS Bioconductor package and illustrate how BASiCS can be used to analyse a single or multiple pre-specified groups of cells.