Table 1.
LNP composition for optimization.
| LNP | Ionizable lipid (mol%) | Trehalose glycolipid (mol%) | Steroid (mol%) | Helper lipid (mol%) | PEG-conjugated lipid (mol%) |
|---|---|---|---|---|---|
| Con-LNP | SM-102 (50) | – | Cholesterol (38.5) | DSPC (10) | DMG-PEG (1.5) |
| D100C | DLin-MC3-DMA (50) | – | Cholesterol (38.5) | DOPE (10) | DMG-PEG (1.5) |
| D050C | DLin-MC3-DMA (25) | TDO (25) | Cholesterol (38.5) | DOPE (10) | DMG-PEG (1.5) |
| D050L | DLin-MC3-DMA (25) | TDO (25) | n-Butyl lithocholate (38.5) | DOPE (10) | DMG-PEG (1.5) |
| T100L | – | TDO (50) | n-Butyl lithocholate (38.5) | DOPE (10) | DMG-PEG (1.5) |
| S025L | SM-102 (12.5) | TDO (37.5) | n-Butyl lithocholate (38.5) | DOPE (10) | DMG-PEG (1.5) |
| S050L | SM-102 (25) | TDO (25) | n-Butyl lithocholate (38.5) | DOPE (10) | DMG-PEG (1.5) |
| S075L | SM-102 (37.5) | TDO (12.5) | n-Butyl lithocholate (38.5) | DOPE (10) | DMG-PEG (1.5) |
| S100L | SM-102 (50) | – | n-Butyl lithocholate (38.5) | DOPE (10) | DMG-PEG (1.5) |
| S050C | SM-102 (25) | TDO (25) | Cholesterol (38.5) | DOPE (10) | DMG-PEG (1.5) |
| S050L′ | SM-102 (25) | TDO (25) | 3-methylpentyl lithocholate (38.5) | DOPE (10) | DMG-PEG (1.5) |
| S050L″ | SM-102 (25) | TDO (25) | iso-Pentyl lithocholate (38.5) | DOPE (10) | DMG-PEG (1.5) |