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. 2024 Mar 12;73(6):926–940. doi: 10.2337/db23-0553

Figure 4.

Figure 4

Histological analysis in the pancreatic tissues of db/db mice receiving FMT from dapagliflozin (Dapa)- or vehicle-treated mice with or without exendin 9-39 (Ex9) for 5 weeks. A: Representative photographs of the entire pancreata immunostained for glucagon and insulin. Scale bar = 2,000 μm. B: Quantification of the islet area per pancreatic area. C: Quantification of the islet number per section. D: Quantification of the small islet number per section. An islet with a cell number less than eight is defined as a small islet. E: Representative photographs of an islet immunostained for glucagon and insulin. Scale bar = 20 μm. F: Quantification of the β-cell area per pancreatic area. G: Quantification of the α-cell area per pancreatic area. H: The ratio of β-cell area to α-cell area. n = 3 sections/mouse and 3 mice/group. Data are mean ± SEM or median (interquartile range). Statistical analysis was performed by one-way ANOVA followed by Dunnett T3 multiple comparisons test in B and C, by Kruskal-Wallis test followed by Dunn multiple comparisons test in F, or by one-way ANOVA or two-way ANOVA followed by Tukey multiple comparisons test as appropriate in D, G, and H. *P < 0.05 (vs. control [Ctrl]-FMT + vehicle).