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. Author manuscript; available in PMC: 2024 May 22.
Published in final edited form as: J Vis Exp. 2022 Jun 16;(184):10.3791/64119. doi: 10.3791/64119

Figure 1: Chemical structure and 1H-NMR spectrum of MethMAL.

Figure 1:

(A) Chemical structure: the MethMAL annealing macromer is composed of 20 kDa 4-arm poly(ethylene glycol) modified with three methacrylamide arms. (B) This structure generates peaks at 5.36 ppm (3) and 5.76 ppm (2) not present in PEG-MAL spectra, and one maleimide arm, which generates a peak at 6.71 ppm (1). The solvent, chloroform, generated a peak at 7.26 ppm, and residual water in this sample generated a peak at 2.2 ppm (labeled on spectra). In the MethMAL spectra, the maleimide peak had an integrated area of 0.27, and the sum of the methacrylamide peaks areas was 0.73 (0.37 + 0.36). The methacrylamide percentage modification was 73% (0.73/(0.27 + 0.73)). This figure is from Pfaff et al.14. Copyright (2021) American Chemical Society.