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. Author manuscript; available in PMC: 2024 May 22.
Published in final edited form as: J Vis Exp. 2022 Jun 16;(184):10.3791/64119. doi: 10.3791/64119

Figure 7: MAP scaffold annealing.

Figure 7:

(A) Schematic of MAP scaffold annealing. When exposed to a photoinitiator and light, the methacrylamide functional groups on the MethMAL macromer undergo a click photopolymerization reaction, which bonds the surfaces of the microgels together. (B) Depiction of a 3D rendering (Imaris) of a two-photon microscope image of MAP microgels (green) annealed together in a 3D puck shape, with dextran (red) in the pores. (C) Depiction of a 3D rendering (Imaris) of a two-photon microscope image showing the porosity of a MAP scaffold that has been perfused with fluorescent 70 kDa dextran (red). Scale bars = (B) 100 µm, (C) 70 µm. Abbreviations: MAP = microporous annealed particle; MethMAL = custom annealing macromer.