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. Author manuscript; available in PMC: 2024 May 22.
Published in final edited form as: J Vis Exp. 2022 Jun 16;(184):10.3791/64119. doi: 10.3791/64119

Table of Materials

Name of Material/ Equipment Company Catalog Number Comments/Description
2-aminoethanethiol hydrochloride Acros Organics AC153770250 MW: 113.61Da
35 mm plate rotor HAAKE P35/Ti Geometry for HAAKE viscometer
4-arm PEG-Maleimide (10kDa) NOF AMERICA Corporation SUNBRIGHT PTE-100MA For microgel precursor solution
4-arm PEG-Maleimide (20kDa) NOF AMERICA Corporation SUNBRIGHT PTE-200MA Molecular weight specific to each batch.
BD Syringe with Luer-Lok Tips Becton Dickinson Disposable plastic syringes
Biopsy punch Mitex MLTX33–31A-P/25 1.5 mm diameter
Chloroform-d Acros Organics AC209561000 For MethMal Synthesis
Collimated LED Light Source ThorLabs M365LP1-C1 365nm
Culture dish (15 cm) Corning CLS430599 150 mm x 25 mm
morpholinium chloride) Oakwood Chemical 151882 MW: 276.72Da
Fluorosurfactant Ran Technologies 008-Flurosurfactant-5wtH-200G 5 weight percent of 008-Flurosurfactant in HFE7500
FreeZone Triad Freeze Dry System Labconco 7400000 Series Lyophilizer
Glass slides Fisher Scientific 12–550-A3 Plain glass slides, uncoated
HAAKE Rheowin viscometer HAAKE
KDS Legato 210 Dual Prong Syringe Pump Kd Scientific
LED Driver ThorLabs DC2200
Lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) Sigma-Aldrich 900889 Photoinitiator
Methacrylic Acid Sigma Aldrich 155721 MW: 86.09Da
Microfluidic device SU8-Si master wafer FlowJem N/A Custom-made, with silanization
MMP-2 degradable crosslinker WatsonBio Sciences Sequence: Ac-GCGPQGIAGQDGCG-NH2
Needles (25 G) BD 305122 Gauge: 0.5 mm
Novec 7500 3M 7100025016 Fluorinated oil
Oxygen Praxair UN1072 Compressed
Peek tubing Trajan Scientific 03–350-523 1/32" Outer Diameter; 0.02" Inner Diameter; 10' Length
PFOCTS (trichloro(1H,1H,2H,2H-perfluorooctyl)silane) Sigma-Aldrich 448931 For surface treatment
Phosphate Buffered Saline Fisher BioReagants BP3994 Diluted to 1X in ultrapure water, pH=7.4
Plasma cleaner Harrick Plasma PDC-001-HP
Razor blade Fisher Scientific 12–640
RGD cell adhesive peptide WatsonBio Sciences Sequence: Ac-RGDSPGGC-NH2
Rheowin software HAAKE Software compatible with HAAKE viscometer
Scalpel blade Bard-Parker 371210 Size: #10
Scalpel handle Bard-Parker 371030 Size: #3
Sodium Chloride Fisher BioReagents BP358–1 MW: 58.44Da
Sylgard 184 silicone elastomer kit DOW Chemical 2065622 Base and curing agent
Triethylamine Fisher Scientific O4884–100 MW: 101.19Da
Tygon tubing Saint Gobain Performance Plastics AAD04103 ID: 0.51 mm
OD: 1.52 mm
Varian Inova 500 Spectrometer Varian NMR
Located in the UVA Biomolecular Magnetic Resonance Facility