Figure 2. 1,25(OH)₂D₃ treatment reduces numbers of CD4⁺ and CD11b⁺ cells in the CNS.

Lumbar spinal cords of 1,25(OH)₂D₃ treated and vehicle EAE mice were harvested at day 30 p.i. for immunostaining. Representative images indicate (A) CD4⁺ T cells and (B) CD11b⁺ microglia/macrophages. Nuclei were stained with DAPI (blue). Quantitative analysis of numbers of (C) CD4⁺ and (D) CD11b⁺ cells per mm² in the spinal cord. Scale bars at c and f are 20 μm in small square and 40 μm in large square, respectively. Data represent mean ± SEM (n=5/group). **P< 0.01. One representative of three experiments is shown.