(A) Splenocytes of 25(OH)2D3 treated and vehicle EAE mice were culture with MOG35-55 for three days and supernatants were collected and analyzed for cytokine production by ELISA. (B) Spinal cord was collected and mRNA expression of IFN-γ, IL-17A, GM-CSF, IL-4 and IL-10 was quantified by RT-PCR. Data represent mean± SEM (n=5/group), *P<0.05, **P< 0.01, and ***p<0.001. One representative of three experiments is shown.