Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (M. musculus) | C57BL/6 J | The Jackson Laboratory, Bar Harbor, ME | RRID:IMSR_JAX:000664 | |
| Genetic reagent (M. musculus) | C57BL/6JN | NIA, Bethesda, MD | NA | |
| Genetic reagent (M. musculus) | Rosa26 loxp-stop-loxp tdTomato Reporter (Ai14) | The Jackson Laboratory, Bar Harbor, ME | RRID:IMSR_JAX:007914 | |
| Genetic reagent (M. musculus) | PdgfraCreERT2 | The Jackson Laboratory, Bar Harbor, ME | RRID:IMSR_JAX:032770 | |
| Antibody | Rabbit polyclonal anti–red fluorescent protein (RFP) | Rockland, Pottstown, PA | 600-401-379, RRID:AB_2209751 | 1:500 |
| Antibody | Rabbit polyclonal anti-Perilipin (D418) | Cell Signaling, Denvers, MA | 3470, RRID:AB_2167268 | 1:200 |
| Antibody | Rabbit polyclonal anti- UCP1 | Specially made by AstraZeneca, Cambridge, UK | NA | 1:2000 |
| Antibody | Rabbit polyclonal Anti-mouse CD142 | Sino Biological, Chesterbrook, PA | R001 | 1:100 |
| Antibody | Goat polyclonal Anti-mouse CD142 | R & D Systems, Minneapolis, MN | AF3178, RRID:AB_2278143 | 1:50 |
| Antibody | Rat monoclonal Anti-mouse CD140a-(PDGFRɑ)-PECy7 | Biolegend, San Diego, CA | 135912, RRID:AB_2715974 | 1:100 |
| Antibody | Rat monoclonal Anti-mouse-CD31 (APC-Fire) | Biolegend, San Diego, CA | 102528, RRID:AB_2721491 | 1:1000 |
| Antibody | Rat monoclonal Anti-mouse CD45-allophycocyanin (APC/Cy7) | Biolegend, San Diego, CA | 103116, RRID:AB_312981 | 1:1000 |
| Antibody | Rat monoclonal Anti-mouse ICAM1-phycoerythrin (PE/Cy7) | Biolegend, San Diego, CA | 116122, RRID:AB_2715950 | 1:100 |
| Antibody | Rat monoclonal Anti-mouse CD26 (DPP-4)- fluorescein isothiocyanate (FITC) | Biolegend, San Diego, CA | 137806, RRID:AB_10663402 | 1:200 |
| Sequence-based reagent | mTbp | PMID:24703692 | NA | F-GAAGCTGCGGTACAATTCCAG R-CCCCTTGTACCCTTCACCAAT |
| Sequence-based reagent | mAdipoq | PMID:24703692 | NA | F-GCACTGGCAAGTTCTACTGCAA R-GTAGGTGAAGAGAACGGCCTTGT |
| Sequence-based reagent | mFabp4 | PMID:24703692 | NA | F-ACACCGAGATTTCCTTCAAACTG R-CCATCTAGGGTTATGATGCTCTTCA |
| Sequence-based reagent | mCidea | PMID:24703692 | NA | F-TGCTCTTCTGTATCGCCCAGT R-GCCGTGTTAAGGAATCTGCTG |
| Sequence-based reagent | mPgc1a | PMID:24703692 | NA | F-CCCTGCCATTGTTAAGACC R-TGCTGCTGTTCCTGTTTTC |
| Sequence-based reagent | mUcp1 | PMID:24703692 | NA | F-ACTGCCACACCTCCAGTCATT R-CTTTGCCTCACTCAGGATTGG |
| Sequence-based reagent | mDio2 | PMID:24703692 | NA | F-CAGTGTGGTGCACGTCTCCAATC R-TGAACCAAAGTTGACCACCAG |
| Sequence-based reagent | mAcly | PMID:31141698 | NA | F-GAGTGCTATTGCGCTTCCC R-GGTTGCCGAAGTCACAGGT |
| Sequence-based reagent | mNpr3 | This Paper | NA | F-TTTTCAGGAGGAGGGGTTGC R-ACACATGATCACCACTCGCT |
| Sequence-based reagent | mNpr1 | MGH PrimerBank | Primer Bank ID: 113930717 c1 | F-GCTTGTGCTCTATGCAGATCG R-CCTCGACGAACTCCTGGTG |
| Sequence-based reagent | mNpr2 | MGH PrimerBank | Primer Bank ID: 118129825 c2 | F-CATGACCCCGACCTTCTGTTG R-CGAACCAGGGTACGATAATGCT |
| Commercial assay or kit | ABI High-Capacity cDNA Synthesis kit | Applied Biosystems, Waltham, MA | 4368813 | |
| Commercial assay or kit | Purelink RNA Mini columns | Invitrogen, Waltham, MA | LT-12183018 | |
| Commercial assay or kit | TSA TMR Tyramide Reagent Pack | Akoya Biosciences, Marlborough, MA | NEL742001KT | |
| Commercial assay or kit | TSA Fluorescein Tyramide Reagent Pack | Akoya Biosciences, Marlborough, MA | NEL741001KT | |
| Commercial assay or kit | Bulls Eye Decloaking Buffer | Biocare, Pacheco, CA | BULL1000 MX | |
| Commercial assay or kit | AbC Total Antibody Compensation Bead Kit | BioLegend,San Diego, CA | A10497 | |
| Commercial assay or kit | Biotium Mix-n-Stain CF647 | Sigma, Burlington, MA | MX647S100 | |
| Commercial assay or kit | PicoPure RNA Isolation Kit | Invitrogen, Waltham, MA | KIT0204 | |
| Commercial assay or kit | Qubit dsDNA High Sensitivity assay kit | ThermoFisher, Waltham, MA | Q32851 | |
| Commercial assay or kit | DNA High Sensitivity Bioanalyzer Chip (Agilent) | Agilent, Santa Clara, CA | 5067–4626 | |
| Software, algorithm | Graphpad Prism | Graphpad, San Diego, CA | RRID:SCR_002798 | |
| Software, algorithm | Adobe Illustrator | Adobe, San Jose, CA | RRID:SCR_010279 | |
| Software, algorithm | Adobe Photoshop | Adobe, San Jose, CA | RRID:SCR_014199 | |
| Software, algorithm | Image J | PMID:22743772 | RRID:SCR_003070 | |
| Software, algorithm | Cell Ranger | 10 x Genomics | RRID:SCR_017344 | |
| Software, algorithm | Seurat | PMID:34062119 | RRID:SCR_016341 | |
| Software, algorithm | bcl2fastq | Illumina | RRID:SCR_015058 | |
| Software, algorithm | Cumulus | PMID:32719530 | RRID:SCR_021644 | |
| Software, algorithm | FACSDiva Softward | Becton Dickinson, Franklin Lakes, NJ | RRID:SCR_001456 | |
| Other | Tamoxifen (Free Base) | Sigma, Burlington, MA | T5648 | Synthetic estrogen receptor antagonist used to activate Cre. |
| Other | Corn Oil | Sigma, Burlington, MA | C8267 | Vehicle solution for tamoxifen. |
| Other | 16% Paraformaldehyde | EMS, Hatfield, PA | 15710 | Fixative used for tissue histology |
| Other | TRIzol | Invitrogen, Waltham, MA | 15596018 | Phenol-based solution used for nucleic acid extraction |
| Other | CL-316,243 | Sigma, Burlington, MA | C5976 | Agonist of Beta3-adrenergic receptor |
| Other | 4’,6-Diamidine-2’-phenylindole dihydrochloride (DAPI), 1:10,000 | Roche, Basel, Switzerland | 10236276001 | Fluorescent stain for DNA/nuclei |
| Other | Bovine Serum Albumin, fraction V, fatty-acid free | Gold Biotechnology, St. Louis, MO | A-421–250 | Protein carrier for small molecules |
| Other | DMEM/F12 | Fisher Scientific, Waltham, MA | 11320033 | Basal cell culture medium |
| Other | Fetal Bovine Serum | Omega Scientific, Tarzana, CA | FB-11, Lot 401714 | For cell culture |
| Other | Primocin | InvivoGen, San Diego, CA | ant-pm-2 | Anti-microbial for cell culture |
| Other | PCR Master Mix, Power SYBR Green | Applied Biosystems, Waltham, MA | 4367659 | Kit for qRT-PCR |
| Other | HBSS, 1 X | Fisher Scientific, Waltham, MA | 14175079 | Hank’s Balanced Salt Solution |
| Other | Dispase II | Roche, Basel, Switzerland | 4942078001 | Enzyme used for adipose tissue digestion |
| Other | Collagenase, Type 1 | Worthington, Lakewood, NJ | LS004197 | Enzyme used for adipose tissue digestion |
| Other | Red Blood Cell Lysis Buffer, 10 x | BioLegend, San Diego, CA | 420302 | For lysing red blood cells during cell isolations |
| Other | Human Insulin, Novolin | Novo Nordisk, Bagsvaerd, Denmark | 183311 | Used for cell culture studies |
| Other | Dexamethasone | Sigma-Aldrich, Burlington, VT | D4902 | Glucorticoid Receptor agonist |
| Other | 3-isobutyl-1-methylxanthine (IBMX) | Sigma-Aldrich, Burlington, VT | I7018 | Chemical used to Increase cAMP levels, used in adipocyte differentiation cocktail |
| Other | Rosiglitazone | Cayman Chemical, Ann Arbor, MI | 11884 | Synthetic PPARgamma activator |
| Other | Indomethacin | Sigma-Aldrich, Burlington, VT | I8280 | Chemical used in adipocyte differentiation cocktail |
| Other | 3,30,5-Triiodo-L-thyronine sodium salt (T3) | Sigma-Aldrich, Burlington, VT | T6397 | Thyroid Receptor agonist |
| Other | isoproterenol | Sigma-Aldrich, Burlington, VT | I6504 | Pan beta-adrenergic receptor agonist |
| Other | Bodipy 493/503 | Invitrogen, Waltham, MA | D3922 | Fluorescent dye for neutral lipids |
| Other | Hoechst 33342 | Thermo Fisher, Waltham, MA | 62249 | DNA stain |
| Other | Protector RNase Inhibitor | Roche, Basel, Switzerland | 3335399001 | Used for RT-PCR |