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. 2024 May 8;629(8013):878–885. doi: 10.1038/s41586-024-07385-1

Fig. 2. Computationally designed IgG antibodies improve Omicron binding and maintain parental thermostability and binding to historical strains.

Fig. 2

a, The parental COV2-2130 (orange circles) and computationally designed antibodies (2130-1-0114-112 in purple triangles, 2130-1-0104-024 in blue diamonds and remainder in black) were assayed for thermal shift (n = 3 technical replicates). Melting temperature (Tm ) calculated based on the Boltzmann method. Data are mean and s.d. b–e, The parental COV2-2130 antibody and computationally designed antibodies (see symbols in a) and cross-reactive positive control antibody S309 (magenta squares) were analysed for relative binding to four SARS-CoV-2 spike RBD variants in the Gyrolab immunoassay: WA1/2020 (b), Delta B.1.617.2 (c), Omicron BA.1 (d) and Omicron BA.1.1 (e). Lines represent a four-parameter logistic regression model fit using GraphPad Prism to each titration, executed without technical replicates. mAb, monoclonal antibody.