Figure 1.

Chemical-genomic screening reveals 49 gene candidates involved in the response to CMX. (a) A schematic depicting the theory underlying chemical genomic screening. From left to right: two parallel genetic pathways (y1 and y2) produce a growth phenotype. When either pathway is mutated, the parallel pathway compensates. When both pathways contain mutations, no compensation can occur resulting in a decrease in growth phenotype. Coupling a single mutation with an inhibitory compound (w) mimics the effect of two mutations, resulting in a decreased growth phenotype. (b) Representative images from large-scale chemical genomic screening. The red arrow indicates a sensitive colony. (c) The sensitivities of each gene candidate to CMX derived from follow up colony size sensitivity analysis.