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. 2024 May 22;14:11695. doi: 10.1038/s41598-024-62563-5

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Small-scale verification of 18 gene candidates to CMX. (a) Spot-test analysis of the 18 candidates in the presence and absence of 75 µM CMX. (b) Quantification of spot-tests in (a). (c) Colony-count analysis of the 18 candidates. Grey bar represents a 20% reduction in colony growth. The red bar represents a 50% reduction in colony growth. (d) GO-analysis of the 8 most-sensitive gene candidates reveals two statistically significant gene families: de novo purine biosynthesis and ribonucleoside biosynthesis.