Figure 3.

The yeast DHFR enzyme Dfr1 is a target of CMX. (a) total RNA was extracted from yeasts subjected to several concentrations of CMX, showing a dose-dependent reduction in rRNA band intensity obtained from a total RNA extraction, quantified in (b). (c) qRT-PCR analysis of DRF1 and HSC82 from RNA extracted from yeasts grown in the presence of increasing concentrations of CMX. (d) β -gal activity of WT and a temperature-sensitive knockdown of Dfr1 in the presence and absence of CMX. (e) Colony-counting analysis of yeasts expressing DFR1 on a plasmid in the presence or absence of CMX. An empty vector not containing Drf1 was used as a control under the same conditions. (f) Using the same strains in (e), both were subject to 30 °C and 37 °C as a control. **Denotes significance values less than 0.01. ***Denotes significance values less than 0.001. The original gel seen in “A” can be found in Supplemental Fig. 1.