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. 2024 May 22;15(5):358. doi: 10.1038/s41419-024-06730-6

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Representative WB of whole cell lysates from HOFs stimulated with A ET-1 at indicated times or B ET-1 (5 min) and/or AMB and/or BQ788 and/or BOS. Tubulin and GAPDH, loading control. Histograms represent the mean ± SD of densitometric analyses of proteins relative to GAPDH; statistics were obtained using One-way ANOVA. C CLSM analysis of HOFs stimulated with ET-1 for 5 min and/or AMB and/or BQ788 and/or AMB + BQ788, stained for active Intβ1 (green), talin1 (magenta) and F-actin (red) detection. Co-localization is shown in merged images (pseudo-color white or yellow). Columns represent the mean ± SD of quantification of Pearson’s correlation between act Intβ1 and talin1; statistics were obtained using one-way ANOVA.