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. 2024 May 22;10:246. doi: 10.1038/s41420-024-02031-1

Fig. 6. CREB1 signaling enhances EZH2’s PRC2 activity.

Fig. 6

A CREB1 signaling activator Fsk (left) (10 µm for 24 h in LNCaP cells) and with ISO (right) (15 µm for 24 h in PC3 cells) activates p-S133-CREB1 and induces bulk H3K27me3 levels. Histone 3 (H3) and beta actin serve as loading controls for nuclear and whole cell lysate proteins, respectively. B p-S133-CREB1 and H3K27me3 levels are induced in PC3 cells expressing constitutively activated CREB1-Y134F mutant cDNA. C CREB1 signaling inhibitor PKI (left) (10 µm for 24 h in 144-13 cells) and with ICI (right) (10 µm for 24 h in NE1.3 cells) reduces p-S133-CREB1 and bulk H3K27me3 levels. D Western blotting indicates that bulk levels of H3K27me3 mark are elevated in LNCaP xenograft tumors from NOD/SCID male mice that were treated with ISO.