Figure 1.

Distinct dynamics of TandemFP expression by 293T cells and human PGP-1 iPSC-derived neural progenitor cells

(A) FACS plots of 293T cell expressing TandemFP from a Tet-ON promoter. Expression was induced by adding Dox to the cell culture medium. Cells were harvested and analyzed after 4 periods of culture—6, 24, 48, and 72 h. Green fluorescence increases strongly by 6 h after induction, with most cells displaying both green and red fluorescence after 72 h.

(B) FACS plots of neural progenitor cells derived from PGP-1 iPSCs expressing TandemFP from the endogenous PAX6 promoter. PGP1-PAX6-tandemFP iPSCs were induced at day (D) 0, and neural progenitors were harvested and analyzed daily. Green fluorescence is detected by D5, however no red fluorescence is detected by D8.