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. 2024 May 7;27(6):109911. doi: 10.1016/j.isci.2024.109911

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© 2024 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

MolTimer2.0 is also expressed by human iPSC-derived neural progenitor cells with appropriate multi-day dynamics

(A) FACS plots of human iPSC-derived neural progenitor cells expressing MolTimer2.0 from the endogenous PAX6 promoter. PGP1-PAX6-MolTimer2.0 iPSCs were induced at D0, and neural progenitors were harvested and analyzed daily. Green and red double-positive cells start to appear from D6 onward, with increasing fluorescence intensity until D10.

(B) Z-projections of confocal imaging stacks of endogenous MolTimer2.0 expression in PGP1-PAX6-MolTimer2.0 neural progenitor cells from D1 to D10. Green and red fluorescence is detected from D6 onward, increasing in intensity until D10.

(C) qPCR analysis of sfGFP, mScarlet, and PAX6 reveals increasing mRNA-level expression of both fluorophore mRNAs together with increased PAX6 expression. Data are represented as mean ± SD.