Table 5.
Characteristics of components of the yeast Sec34p/Sec35p complex
| Gene | ORF | Size of protein | Organisms with homologsa | Difference in mobility of Sec34p/Sec35p in mutant relative to wild typeb | Null phenotype (24°C) | Two-hybrid interactionsc |
|---|---|---|---|---|---|---|
| SEC34 | YER157w | 93 kD | H,D,A,S,C,Y | Not determined | Visible microcolonies | Sec34p-Sec35p |
| SEC35 | YGR120c | 32 kD | Y | 3.0, 5.0 (Sec34p) | Visible microcolonies | Sec35p-Sec34p |
| Sec35p-Sec37p | ||||||
| Sec35p-Sec38p | ||||||
| SEC36/COD3 | YGL223c | 48 kD | Y | 2.5 | Visible microcolonies | Sec36p-Sec37p |
| SEC38/SGF1/COD1 | YPR105c | 99 kD | H,D,A,S,C,Y | 4.5 (Sec34p) | No visible growth | Sec38p-Sec35p |
| 6.5 (Sec35p) | ||||||
| SEC37/COD2 | YNL041c | 97 kD | H,D,A,S,C,Y | 1.2 | Wild-type growth | Sec37p-Sec35p |
| Sec37p-Sec36p | ||||||
| COD4 | YNL051w | 46 kD | H,D,A,S,C,Y | 1.3 | Wild-type growth | |
| COD5 | YGL005c | 32 kD | Y | 0.5 | Wild-type growth | |
| DOR1 | YML071c | 70 kD | H,D,A,S,C,Y | 1.4 | Wild-type growth |
a
Organisms are indicated as H. sapiens (H), D. melanogaster (D), A. thaliana (A), S. pombe (S), C. elegans (C), and at least one other budding yeast (Y).
b
Summary of data from Figures 5, 6, 11, 12, and 13. Mobility differences are expressed as the volume (ml) difference between the peak of Sec34p and Sec35p elution from a Suprose 6 column in mutant extracts relative to corresponding wild-type extracts.
c
Summary of data from VanRheenen et al. (1999), Uetz et al. (2000), Kim et al. (2001), and Ito et al. (2001).