Fig. 2.

LC-MS quantification of oxysterols in bovine lens lipid membranes after exposure to IR. A) 7β-hydroxycholesterol, B) 7-ketocholesterol in, C) 5, 6-epoxycholesterol, D) total oxysterols were calculated by combining levels for 7β-hydroxycholesterol, 7-ketocholesterol and 5, 6-epoxycholesterol. Levels of these oxysterols were measured in the membrane fractions from the bovine lens cortex (Cortex) and nucleus (Nucleus) that had been exposed to 0, 5 and 50 Gy IR. General Linear Model Analysis of Variance followed by Tukey pairwise comparison post hoc test using location and dose as independent factors was applied for statistical analysis, *p< 0.05, **p<0.001 compared to untreated control and # p<0.01 cortex compared to nucleus. n = 3,.