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Effect of trans-chalcone treatment on the mRNA values of TNF-α (A), IL-1β (B), IL-6 (C), IL-17 (D), COX-2 (E), iNOS (F), and IL-10 (G) in arthritic rats determined by real-time PCR. Results are given as means with SEM (n = 6). Significance levels: ### indicates p < 0.001 in contrast to the normal control ** denotes p < 0.01 and *** denotes p < 0.001 in comparison to the arthritic control. Statistical tests used were one-way ANOVA and the Dunnett tests.
