Table 2. Binding Interaction of Trans-Chalcone and the Cocrystallized Ligand with Different Target Proteins Determined through Docking Analysis.

			H-bond interactions
target protein	phytochemical/ligand	binding energy (kJ/mol)	score (%)	distance (°A)	residue amino acid	arene–arene interactions	arene–cation interactions
TNF-α	trans-chalcone	–8.774	25	2.52	Gly 121	Tyr 59
	cocrystallized ligand	–13.410	92	1.78	Tyr 119	Tyr 119
IL-17	trans-chalcone	–9.505	79	2.29	Leu 97
	cocrystallized ligand	–10.825	31	1.23	Leu 97
COX-2	trans-chalcone	–9.071	52	2.64	Tyr 355
	cocrystallized ligand	–12.171	71	1.79	Ser 530
			91	2.07	Ser 353
iNOS	trans-chalcone	–8.718	11	3.15	Ala 351
			73	2.4	Tyr 347
	cocrystallized ligand	–11.819	31	1.83	Trp 372
			13	2.23	Tyr 347
			14	2.67	Gly 371