Table 4.
Primes used for the detection of C. difficile toxin genes
| Target gene | Prime | Oligonucleotide sequences (5’ – 3’) | Product length (bp) |
|---|---|---|---|
| tcdA a |
tcdA-F tcdA-R |
AGATTCCTATATTTACATGACAATAT GTATCAGGCATAAAGTAATATACTTT |
369 |
| tcdB a |
tcdB-F tcdB-R |
TGATGAAGATACAGCAGAAGC TGATTCTCCCTCAAAATTCTC |
688 |
| tcdC a |
tcdC-F tcdC-R |
AAAAGGGAGATTGTATTATGTTTTC CAATAACTTGAATAACCTTACCTTCA |
479 |
| tcdR b |
tcdR-F tcdR-R |
AAAAGCGATGCTATTATAGTCAAA CCTTATTAACAGCTTGTCTAGAT |
300 |
| tcdE b |
tcdE-F tcdE-R |
GTTTAAGTGCAATAAAAAGTCGTA GGTAATCCACATAAGCACATATT |
262 |
| cdtA a |
cdtA-F cdtA-R |
GGGAAGCACTATATTAAAGCAGAAGC CTGGGTTAGGATTATTTACTGGACCA | 200 |
| cdtB a |
cdtB-F cdtB-R |
TTGACCCAAAGTTGATGTCTGATTG CGGATCTCTTGCTTCAGTCTTTATAG |
260 |
a: The primers used in the study were designed by Primer-BLAST
b: The primers used in the study were from Reference 48