Fig. 7.

Effects of BDNF overexpression in hippocampal CA1 glutamergic neurons on BDNF expression, synaptic plasticity and neuronal excitability. A Fluorescence images showed that the AAV-BDNF vector was effectively expressed in the CA1 region of the hippocampus. B Representative Western blot protein bands. C Quantitative results show that the level of BDNF in hippocampal CA1 region of BDNF-overexpression mice was increased **p < 0.01 (n = 8). BDNF expression in each sample was normalized to that of GAPDH. The BDNF level of control mice was set to 1 for quantification. D Representative images of BDNF and CaMKIIα. Scale bar, 100 μm. E Quantitation of the co-labeling rate of BDNF and CaMKIIα **p < 0.01 (n = 6). F Representative images of c-Fos and CaMKIIα. Scale bar, 100 μm. G Quantitation of the co-labeling rate of c-Fos and CaMKIIα *p < 0.05 (n = 6). H Profile images of Golgi staining of neurons in the hippocampal CA1 region. 20 × with camera tracings and 60 × for spine counting. Scale bar, 100 μm for 20 ×; 10 μm for 60 ×. I Quantification of dendritic bifurcations *p < 0.05, **p < 0.01 (n = 18). J Quantitation of the dendritic spine density **p < 0.01 (n = 18). K Quantitation of the total dendritic length **p < 0.01 (n = 18)