Figure 6.

Mutation of thr-31 in Tlg1 and ser-90 in Tlg2 is sufficient to restore endocytosis, but not exocytosis, to snc yeast. (A) Mutation of thr-31 in Tlg1 and ser-90 in Tlg2 restores endocytosis to snc yeast. snc cells (JG8) expressing a control plasmid (snc) or overexpressing SNC1, TLG1, TLG2, TLG1^ala31, or TLG2^ala90 were grown, shifted to glucose-containing medium for 24 h, and labeled with FM4-64. Cells were visualized using Nomarski optics (Light) or via the rhodamine channel (FM4-64). (B) Mutation of thr-31 in Tlg1 and ser-90 in Tlg2 does not restore growth to snc yeast. snc cells (JG8) expressing a control plasmid (snc) or overexpressing SNC1, TLG1^ala31, TLG2^ala90, SSO1^ala79, or both SSO1^ala79and TLG1^ala31 or SSO1^ala79 and TLG2^ala90 were grown, shifted to glucose-containing medium for 24 h, diluted serially and plated onto galactose-containing medium (SC+GAL) at 26°C, glucose-containing medium (SC+GLU) at 26, 35.5, or 37°C, or amino acid-rich medium (YPD) at 26°C. Cells were grown for 48 h.