Table 2.
Plasmids created for this study
| Plasmid name | Gene expressed | Vector | Sites of cloning | Oligo namea |
|---|---|---|---|---|
| pGST-SIT4 | SIT4 | pGEX4T-3 | EcoRI and XhoI | Sit4-f, Sit4-r |
| pGST-TLG11–206 | TLG11–206 | pGEX4T-3 | EcoRI and SalI | Tlg1-f, Tlg1-r |
| pGST-TLG21–317 | TLG21–317 | pGEX4T-3 | EcoRI and SalI | Tlg2-f, Tlg2-r |
| pADH-mycTLG1 | myc-TLG1 | pAD6 | SalI and SacI | Tlg1-f, Tlg1-r |
| pADH-mycTLG2 | myc-TLG2 | pAD6 | SalI and SacI | Tlg2-f, Tlg2-r |
| pADH-mycTLG1ala31 | myc-TLG1ala31 | pAD6 | SalI and SacI | Tlg1A31-f, Tlg1A31-r |
| pADH-mycTLG2ala6 | myc-TLG2ala6 | pAD6 | SalI and SacI | Tlg2A6-f, TlgA6-r |
| pADH-mycTLG2ala15 | myc-TLG2ala15 | pAD6 | SalI and SacI | Tlg2A15-f, TlgA15-r |
| pADH-mycTLG2ala54 | myc-TLG2ala54 | pAD6 | SalI and SacI | Tlg2A54-f, TlgA54-r |
| pADH-mycTLG2ala90 | myc-TLG2ala90 | pAD6 | SalI and SacI | Tlg2A90-f, TlgA90-r |
| pADH-mycTLG2ala101 | myc-TLG2ala101 | pAD6 | SalI and SacI | Tlg2A101-f, TlgA101-r |
| pADH-mycTLG2ala138 | myc-TLG2ala138 | pAD6 | SalI and SacI | Tlg2A138-f, TlgA138-r |
| pGEM-SIT4 | SIT4 | pGEM-T | SalI | Sit4-2-f, Sit4-2-r |
| YEpTSIT4b | SIT4 | pRS424 | SalI | |
| pTADH-mycTLG1c | myc-TLG1 | pRS424 | BamHI | |
| YCpKSTE2-GFPd | STE2-GFP | pRS314STE2-GFP | EcoRI | |
| pKADH-SNC1e | SNC1 | pADH-SNC1 | ClaI |
a
Oligonucleotide sequences will be provided upon request.
b
This plasmid was created by subcloning a SalI fragment from pGEM-SIT4 into pRS424.
c
This plasmid was created by subcloning a BamHI fragment from pADH-mycTLG1 into pRS424.
d
This plasmid was created by subcloning a blunt-ended EcoRV and SalI fragment encoding kanr into pRS314STE2-GFP.
e
This plasmid was created by subcloning a blunt-ended EcoRV and SalI fragment encoding kanr into pADH-SNC1.