Fig 1.

Elucidation of the PmrAB regulon in A. baumannii by RNA-seq. (A) Genes with log₂ (fold change) ≥ 2 and P value < 0.05 compared with the WT strain were considered significantly upregulated, and Venn diagrams were generated to illustrate this. The 18 genes common to the pmrA and B mutants but not to ΔpmrA-CM012S were identified as regulons. (B) A spatial enrichment distribution plot of CM012S (pmrB-activating mutant strain) is presented. The vertical axis indicates log₂ (fold change), and the horizontal axis represents gene loci in the genome sequence. Red dots indicate regulon genes (18 genes). (C) List of the upregulated 18 regulon genes in the ATCC 19606 pmrAB-activating mutant strain is provided, which shows the locus tag of the ATCC 19606 strain (GenBank accession number: AP025740.1), its product, gene name, and expression levels of each gene in each strain, depicted on a heatmap.