Fig. 1.

The principle of allogeneic hematopoietic stem cell transplantation after short-term pre-incubation of the graft by anti-human CD4 antibodies (MAX.16H5 IgG₁) or treatment of recipient Balb/c^wt mice at day of transplantation (day 0) is shown. Allogeneic hematopoietic stem cell grafts were incubated with MAX.16H5 IgG₁ 2 h before transplantation into Balb/c^wt mice or recipient mice were treated with 15 μg/g body weight at day 0. Engraftment and hematological (WBC subsets) and immunological reconstitution (murine cd4, human CD4, murine cd8, HLA-DR3, and H2K^b) were measured weekly (four independent transplantation experiments, one experiment for direct antibody application, at least four animals per group in the transplantation experiments and three animals per time point in the antibody application experiments)