Fig. 6.

a Relative FoxP3 expression 46 days after transplantation of 2 × 10⁷ MAX.16H5 IgG₁ pre-incubated BM cells and 2 × 10⁷ splenocytes from TTG-C57Bl/6 mice into Balb/c^wt mice (one experiment, n = 1). The relative FoxP3 expression showed an increase after allogeneic transplantation 46 days after transplantation in liver, spleen, and gut of a transplanted animal. b–d Tolerance transfer by transplantation of 2 × 10⁷ BM cells and 2 × 10⁷ splenocytes from GvHD-free chimeric TTG-Balb/c mice into third party Balb/c^wt recipient mice. BM cells and splenocytes (2 × 10⁷ BM+ 2 × 10⁷ splenocytes) were transplanted into third party Balb/c^wt mice without pre-incubation with MAX.16H5 IgG₁ (n = 4, one experiment). b The relative FoxP3 expression showed a continuous increase of donor derived (human CD4⁺/FoxP3⁺) T-cells after allogeneic transplantation from Balb/c-TTG chimeras in third party Balb/c^wt recipients compared to the controls (n = 4, one experiment). c Flow cytometric analysis 60 days after transplantation showed engraftment of TTG donor cells. The expression of human CD4⁺, HLA-DR⁺, and cd8⁺ cells significantly increased in these animals. The loss of murine cd4-expressing cells also indicated the engraftment of the donor cells (n = 4, one experiment). d Leukocytes (WBC) from surviving Balb/c recipient mice showed recovery to the initial levels (n = 4, one experiment) after 120 days. Total body irradiation from control mice led to a survival of 0 % (one experiment, n = 3)