Fig. 1.

Cerebellar cortex structure in organotypic slice cultures. a Calbindin-positive PC (red), neurofilaments (green), and nuclei (blue) display the three-layered cortex structure, with organotypic organization of neuronal input and output. b Dendritic trees of PC extend into the molecular layer, while a line-shaped arrangement of PC-somata characterizes the Purkinje cell layer. Axons transit the granule cell layer. c PC-dendrites are covered with numerous dendritic spines, visualized by microinjection of pEYFP-tagged actin in single PC. Scale bars (a, b) 100 μm, (c) 10 μm