Fig. 3.

Incubations for 1 day (1d). a, b Spine number in calbindin-labeled PC (red, 7 div) in controls (a) and after progesterone incubation for 1 day (b). c Quantitative analysis of PC spine number in cell cultures treated with various drugs for 1 day at different developmental ages; error bars SD. d Total spine number and spines number of PSD95-positive functional spines counted on the 40-μm dendritic tree in PC 15 after 15 div; error bars SD. e YFP-actin labeled dendritic spines (green) and PSD95-labeled functional synapses (red) are also co-localized after progesterone incubation. f Semi-quantitative reverse transcription-PCR analysis of the physiological PR-A and PR-B mRNA distribution in untreated cerebellar slices after 7 (1), 15 (2), and 30 (3) div. g Semi-quantitative reverse transcription-PCR of slice cultures treated with progesterone (4) and mifepristone (5) for 1 day (1d). White arrows indicate dendritic spines, black arrows the postsynaptic density. Scale bars: (a, b) 5 μm (e) 5 μm