Fig. 2.

Effect of GCN2 extinction by siRNA on HIV-1 infectivity. a Hela P4 cells were transfected with increasing quantities of GCN2 siRNA or control siRNA. GCN2 was detected by Western blot 48 h after transfection with anti-GCN2 antibodies. Control of input was performed with anti-actin antibodies. b HeLa P4 cells were transfected with 2.5 pmol of siRNA. Cells were infected with HIV-1 24 h post-transfection. Infectivity was measured 24 h post-infection (48 h post-transfection) after transfection of cells with GCN2 siRNA (dark grey bar), control siRNA (dark bar), or without transfection (light grey bar). P-values (using Student’s test) calculated from three experiments were 0.038 for GCN2 siRNA transfection versus untransfected cells, 0.015 for GCN2 siRNA transfection versus control siRNA, and 0.43 for control siRNA versus untransfected cells