Fig. 4.
Effect of GCN2 silencing on translation. HeLa P4 cells were transfected with 20 pmol of GCN2 siRNA or control siRNA as described in Methods. Cells were infected with HIV-1Laï (M.O.I. 0.3) 72 h post-transfection for 4 h, and neo-synthesized proteins were labeled as described in Methods. Appropriate protein loading (25 μg) was checked by Coomassie blue. staining of the gels. Translation in infected cells and non-infected cells is shown in (a) Verification of protein loading after Coomassie staining (b) is shown in infected (+) and non-infected (−) cells. MW, molecular weight standards