Fig. 2.

Lysozyme fibrils, but not amorphous aggregates, induce IL-1β secretion. a, b PMA-primed THP1 cells were incubated for 6 h with the indicated amounts of either lysozyme fibrils, amorphous aggregates or monomers, ATP (2 mM) or Nigericin (10 μM). Cell supernatants were analyzed by ELISA (a) or immunoblotting for IL-1β (b, upper panel) and for activated caspase-1 (casp-1) (b, lower panel); c LPS-primed mouse BMDMs were stimulated for 21 h with the indicated amounts of either lysozyme fibrils, monomers, or amorphous aggregates, or transfected with poly(dA:dT) (5 μg/ml), and cell supernatants were analyzed for IL-1β by ELISA. n = 3, represented as mean ± standard deviation (SD)