Fig. 3.

a Whole-cell current recorded from an HEK293 cell expressing homomeric α1 GlyRs showing the phases of an ensemble current in response to a saturating concentration of glycine. b Single channel currents recorded from outside-out patches expressing α1 GlyRs. The records show short bursts of activity at low ligand concentrations (top), which group into clusters at saturating ligand concentrations. The clusters are separated by non-conducting periods where the channel adopts desensitized states (bottom)