Fig. 1.

The hBD2 interaction with human potassium channels transfected in HEK293 cells. a Inhibition of Kv1.3 channel currents by scorpion toxin ChTX. 48.6 ± 2.2 and 69.9 ± 1.2 % blockage by 1 nM and 10 nM ChTX, respectively. b Inhibition of Kv1.3 channel currents by hBD2. 69.4 ± 2.3 % block by 100 pM hBD2. c Concentration-dependent inhibition of Kv1.3 channel currents by hBD2. The IC₅₀ was calculated to be 22.0 ± 7.1 pM. d–f 1 μM hBD2 inhibited potassium currents of 16.5 ± 0.2 % for hKv1.2 (d), 95.7 ± 0.6 % for Kv1.3 (e), 12.5 ± 0.9 % for KCNQ1 (f), respectively. The black and red lines represent the control and measured currents in the absence and presence of hBD2, respectively. Each channel was tested at least three times. Results were shown as the mean ± SE