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. 2024 Apr 22;23(5):e14111. doi: 10.1111/acel.14111

FIGURE 2.

FIGURE 2

Flow cytometry analyses and real‐time RT‐PCR after siPLIN2 and OA‐BSA treatment. (a) BODIPY 493/503 fluorescence mean intensity quantification after siPLIN2 treatment in three hDFs samples from young donors (Y). (b and c) Representative fluorescence microscopy images of hDFs treated with siPLIN2 or scramble siRNA (SCR) and stained with BODIPY 493/503 (green). (d and e) Real‐time RT‐PCR analysis of PLIN2 expression after siPLIN2 and OA‐BSA treatment in hDFs from (d) six young and (e) five old donors. (f–h) Flow cytometry analysis of BODIPY 493/503 intensity after siPLIN2 and OA‐BSA treatment, in hDFs from (g) three young and (h) three old donors. Data are expressed as mean ± SE. Student's t test was applied.