Fig. 1.

Early developmental defects of the cardiac outflow tract in mice with neural crest-specific SoxC gene ablations. Macroscopic appearance of the anterior heart pole (a–f) and hematoxylin-eosin stainings of corresponding consecutive serial sections (horizontal plane) (g–o) from wild-type embryos (Wt) (a, g, h, i) at 13.5 dpc or age-matched embryos with Wnt1::Cre-mediated, neural crest-specific SoxC gene ablations. Analyzed genotypes included: Sox11 ^ΔWnt1 (Sox11Δ) (b, c, j, k, l), Sox4 ^ΔWnt1 (Sox4Δ) (d), and Sox4 ^ΔWnt1 Sox11 ^ΔWnt1 (Dko) (e, f, m, n, o). Observed outflow tract abnormalities included DORV in Sox11 ^ΔWnt1 and Sox4 ^ΔWnt1 Sox11 ^ΔWnt1 embryos (c, e, j, k, l) as well as CT in Sox4 ^ΔWnt1 Sox11 ^ΔWnt1 embryos (f, m, n, o). Ao aorta, CT common arterial trunk, LA left atrium, RA right atrium, RV right ventricle, PT pulmonary trunk. Scale bars 500 μm