Fig. 7.

Analysis of marker gene expression of cardiac neural crest in mice with neural crest-specific SoxC gene ablations. Co-immunohistochemistry was performed at 10.5 dpc on embryos carrying a combination of Rosa26 ^{stopflox-EYFP} and Wnt1::Cre alleles (Wt) (a–d) or carrying the Rosa26 ^{stopflox-EYFP} on a Sox4 ^ΔWnt1 Sox11 ^ΔWnt1 background (Dko) (e–h). Antibodies directed against EYFP were used to detect cells of neural crest origin (green). Actin microfilaments were detected by fluorescently labeled phalloidin (a, b, e, f) and NG2 expression by specific antibodies (c, d, g, h) (both in red). Nuclei were counterstained with DAPI (blue). The area shown at higher magnification in b, d, f, h is boxed in a, c, e, g. Scale bars 100 μm