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Silencing by unpaired DNA mechanism. The crosses used to generate the flies are provided in the Supplementary Methods S3. The flies were checked for genotype and the consistency of EGFP expression/non-expression. Three representative flies were then selected for photography under the same conditions and the same fluorescence microscope parameter. EGFP was silenced in the heterozygous flies (a) but not in the homozygous ones (b)
