Table 2.

SLAP-interacting proteins

Name	Method and domain involved	Functions	References
CBL	In vitro GST pull-down, co-transfection in COS7 cells, phosphorylation-independent interaction, associates through C-terminal hydrophobic region	positive regulation of NFAT-AP1 activity	[14]
	Co-immunoprecipitation in transfected Jurkat T cells	Negative regulation	[33]
	Co-immunoprecipitation, independent of PDGF stimulation	Negative regulation	[45]
CD247/CD3ζ/TCRζ	Co-immunoprecipitation, TCR activation, indirect interaction, associates through SH2 domain	Positive regulation of NFAT-AP1 activity	[14]
	Co-immunoprecipitation in Jurkat T, associates through SH2 domain	Down-regulates TCRζ	[32]
CD79A/Igα	Co-immunoprecipitation, TCR activation, associates through SH2 domain	Negative regulation	[29]
EPHA2/ECK	In vitro GST pull-down, ligand stimulation-dependent associates through SH2 domain	Unknown	[6]
		Tumor suppressor functions	[44]
FLT3	Co-immunoprecipitation in COS1 cells, ligand stimulation	Positive regulation of AKT, ERK, p38 signaling	[22]
KIT	Co-immunoprecipitation in transfected COS1 cells, endogenous in P815 cells, ligand stimulation, associates through SH2 domain	Negative regulation	[23]
PDGFRB	Overexpression in COS7 cells, endogenous in NIH3T3 cell line, ligand stimulation-dependent, associates through SH2 domain	SLAP tyrosine phosphorylation, negatively regulates mitogenesis	[1]
	Binding was not been determined	Strongly inhibits PDGF response	[21]
	Co-immunoprecipitation, ligand dependent	Negative regulation	[45]
SYK	Co-immunoprecipitation in transfected COS7 cells, associates through SH2 domain	SLAP dimerization is independent of SYK or LCK activity, required for positive regulation of NFAT-AP1 activity	[14]
ZAP70
LAT/pp36
SLAP	Forms homodimers through C-terminal region
VAV1	GST pull-down and immunoprecipitation in SLAP-transfected Jurkat TAg cells	Inhibited TCR-induced NFAT activation	[13]
LCK
SLP76
EPOR	Co-immunoprecipitation in overexpressed 293T cells and EI-11 erythroblasts	Negative regulation	[24]
UB4EA	Through the SH3 domain	Tumor suppressor functions	[44]
GM-CSFRα	GST pull-down, both SH3 and SH2 domains are involved	Regulates monocytic dendritic cell maturation	[41]
CSF1R	GST pull-down, CSF1-dependent	Unknown	[47]