Fig. 1.

Spatial organization of tumor cells, T cells and ECM components in human lung and ovarian carcinomas. a In a human lung tumor, tumor cells (brown, stained for cytokeratin) form well-delineated tumor islets surrounded by a stroma (blue). b In a human lung tumor, T cells (brown, stained for CD3) are preferentially distributed within the tumor stroma and very rarely in contact with tumor cells. Dotted white lines denote tumor islets. c In a human lung tumor, thick and linear fibronectin fibers (brown, stained for fibronectin) surround tumor islets denoted by dotted white lines. d In a human ovarian tumor, tumor cells (red, stained for EpCAM) are surrounded by straight and parallel collagen fibers (green) detected by SHG on a two-photon microscope. Bar 100 μm