Fig. 5.

Gold nanospheres and nanoflowers reorganize the nuclear membrane and lamina. MCF7 cells incubated with vehicle, small gold nanospheres (small spheres), gold nanoflowers (flowers), or big gold nanospheres (big spheres) were analyzed by indirect immunofluorescence with antibodies against nuclear pore complexes (mab414), the nuclear lamina (lamin A) or the cytoplasmic protein lactate dehydrogenase [44]. The staining was carried out for controls, heat-shocked cells and after a 2-h recovery period. Panels show single staining or merged images; size bar is 20 μm