Fig. 8.

Small gold nanospheres and gold nanoflowers reorganize the nucleolar subcompartments that synthesize RNA de novo. MCF7 cells were incubated with vehicle or GNPs at 37 °C as described for Fig. 7. Confocal stacks were acquired and surface rendering was performed to visualize new RNA production. DNA was stained with DAPI; newly synthesized RNA was labeled with EU. Note that small gold nanospheres and gold nanoflowers disturb the nucleolar subcompartments that transcribe rDNA