Table 1.

Summary of ARF1 ala-scan mutations

arf1 Allele	Amino acid substitutions	DNA sequence changesa	Diagnostic restriction sites
101	G2A	GGT	Gain HaeIII
		GCC
102	K7A	AAG	Lose DdeI
		GCA
103	K16, E17, R19	AAAGAAATGCGT	Gain BbvI
		GCAGCAATGGCT
104	D26	GAT	Gain HpaII
		GCC
105	K30	AAG	Gain BanI
		GCC
106	K36, K38, K41	AAGTTGAAATTGGGTGAA	Gain BsgI
		GCCTTGGCATTGGGTGCA
107	E54	GAA	Noneb
		GCC
108	K59	AAG	Noneb
		GCA
109	D67	GAT	Gain HaeIII
		GCC
110	D72, R73, R75	GACAGAATTAGA	Lose BglII
		GCCGCAATTGCA
111	R79, H80	AGACAC	Lose BsmA1
		GCAGCC
112	R83, E86	AGAAACACTGAA	Gain PstI
		GCAAACACTGCA
113	D93, D96, R97	GATTCTAACGATAGA	Gain SfaNI
		GCTTCTAACGCTGCA
114	R99	CGT	Lose BstUI
		GCT
115	E102, R104, E105	GAAGCTAGAGAA	Gain Pst1
		GCTGCTGCAGCA
116	R109	AGA	Gain NlaIII
		GCT
117	E113, D114, E115	GAAGATGAATTGAGA	Gain PvuII
	R117	GCAGCTGCATTGGCA
118	K127, D129, E132	AAGCAAGATTTGCCAGAA	Gain HindIII
		GCTCAAGCTTTGCCAGCA
119	E138, E141, K142	GAAATCACTGAAAAA	Gain PstI
		GCAATCACTGCAGCA
120	H146, R149, R151	CATTCTATTAGAAACCGT	Gain SfaNI
		GCATCTATTGCAAACGCT
121	E164	GAA	Gain BspMI
		GCA
122	E168, E171	GAAGGTTTGGAA	Gain Bgl I
		GCCGGTTTGGCA
123	K178	AAA	Gain HaeIII
		GCC

^a Mutant sequence is below the corresponding wild-type sequence. Altered codons are in bold; altered nucleotides are underlined. 

^b No restriction site changes were introduced by the mutation. TaqMan (see MATERIALS AND METHODS) was used to identify mutants.