Table 3.
Plasmids used in this study
| Plasmid | Description | Source |
|---|---|---|
| pUC119 | pUC19 with M13 origin of replication | Sambrook et al., 1989 |
| pJJ283a | LEU2 in pUC18 | Jones and Prakash, 1990 |
| pRB1291 | ARF1 in pUC19 | This laboratory |
| PRB1297 | ARF1 in Ycp50 | Stearns et al., 1990 |
| pRB2925 | ARF1 (EcoRI-PstI) from pRB1291 in pUC119 | This study |
| pRB2927 | ARF1-LEU2 in pUC119. BamHI-XbaI (LEU2) of pJJ283 Klenow-filled and inserted into XbaI site in pEC1 just downstream of ARF1. LEU2 and ARF1 are oriented in the same direction. | This study |
| pRB2816–2861 | pRB2927 mutagenized by site-directed mutagenesis to give two isolates of each Ala-scan mutation arf1-101 through arf1-123 | This study |
a
PJJ283 replaces PJJ252.