Fig. 5.

Western blot analysis of global MBD and histone levels. KLE, HEC-1B and Ishikawa cells were treated under the conditions described in Fig. 3. Protein extracts were isolated and quantified as described under “Materials and methods”. Cellular levels of MBDs and total or acetylated histones were detected using specific antibodies. The results of β-actin (43 KDa) provide controls for protein loading