Fig. 5.

Analysis of O₂ influence on development of BLBP-GFP-positive cells. a Visualisation of BLBP-GFP-positive, progenitor cells (SOX2) and proliferating cells (Ki67) in an organotypic brain slice (E12, 3 DIV) by immunofluorescence (IF). b Co-localisation of BLBP-GFP, SOX2 and Ki67 at 1 and 3 DIV showing that a large portion of the GFP expressing cells are actively dividing (Ki67+) and remain in an undifferentiated state (SOX2+). c Examples of images of regions with overlapping BLBP-GFP and PA2 distributions (intensity, lifetime and overlay) showing ROIs used for quantification. d Profiles of BLBP-GFP expression and corresponding O₂ levels on 1 and 3 DIV, determined for such overlapping regions. Scale bar in µm