Fig. 3.

AnkyrinG expression is necessary to localize β-catenin-pS33/37/T41 to the AIS. a, b 5-DIV hippocampal neurons nucleofected with GFP plasmids expressing scrambled shRNA (a) or ankyrinG shRNA (b). Neurons were immunostained with ankyrinG (red) and pS33/37T41-β-catenin (green) antibodies. Enlarged views of the AIS are shown in right panels in gray scale. Arrows indicate the position of the soma. c The graph represents the percentage of neurons with presence of ankyrinG (gray bars) or β-catenin-pS33/37/T41 (black bars) at the AIS in neurons nucleofected with scrambled or ankyrinG shRNAs. Data represent the mean ± SEM of three independent experiments (50 neurons/experimental condition and experiment). ***p < 0.001, t test. Scale bar 50 µm